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1 Surname________________ Name ________________registration (matricola)_____________ Technologies for regenerative medicine - Prof. Manuela T. Raimondi Examination theme - 7 February 2017 Read the text below and answer, point by point, to the related questions. Stomach tissue engineering. Consider a therapy for stomach regeneration in patients with a full -thickness wall lesion due to a gastrotomy. The therapeutic strategy consists in the obtainment of stomach cells, their expansion and seeding on a polymeric membrane, the (ectopic) implantation of the cellularised construct in the patient peritoneum for several weeks, and finally its orthotopic implantation at the stomach lesion site. Consider the cellularized construct during its ectopic implantation period inside the peritoneum. The polymeric membrane is seeded on both sides with cells. The cell volumetric consumption is a constant equal to V . The peritoneum microenvironment surrounding the construct is under venous oxygen tension, with a conce ntration equal to cv. The membrane is impermeable to oxygen and its thickness is constant and equal to s. Assume that the thickness of the cell layer is constant and equal to s/2. The oxygen diffusion coefficient in the cell layer is D. This therapy, tested on the first ten human patients, ha s shown adequate safety for the treatment of lesions of maximum size not exceeding 10x10 cm. Answer briefly to the questions in the table (Points 5/33): What is the therapeutic product? The cellularised construct ready for orthotopic implantation What are the elements that identify the therapeutic product as a PTC? The main therapeutic agent are the cells, obtained after "non-minimal manipulation" consisting in cell isolation and cell expansion In what stage of the regulatory process…