Skin

Pagina 1

Skin burns tissue regeneration Among the conventional therapy for skin burns of grade II-III in clinical use, there is the regeneration of epidermis and dermis (for lesions < 30 cm2 in dimensions). In a new research, the lesion is surgically prepared and covered with a new biodegradable synthetic membrane, that progressively degrades and is repopulated by fibroblasts and endothelial cells that migrate from the underlying tissue. Keratinocytes then migrate from the peri-lesional tissue to re- epithelialize the lesion . Finally, the silicone membrane, that was placed as a protection cover, progressively detaches from the lesion. Epithelial cells (composed by keratinocytes and their progenitors) were isolated and then expanded in culture flasks. In order to promote their proliferation, they were seeded on the polymeric membrane according to two methodologies: 1. The membrane with a diameter of 1 cm is placed into each well of a six -well plate. Then, culture medium containing cells is dropped on the surface of the polymeric membrane in each well in order to permit adequate seeding. The device is maintained at 37°C. 2. Cells in a normal culture medium are sandwiched between two polymeric membranes; this structure is placed on a temperature-responsive dish at 37°C. After the in vitro culture and expansion, rats were subjected to a surgical procedure during which a circular full-thickness layer of skin measuring 1 cm in diameter was removed. The polymeric membranes populated with epithelial cells were implanted in rats and placed so that the defect was secured using a polydioxanone suture. Answer briefly to the questions in the table: What is the therapeutic product? Cells (keratinocytes and their progenitors) grown on the polymeric membrane What are the elements that…