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Full exam for Technologies for Sensors and Clinical Instrumentation in the Biomedical Engineering degree programme at Politecnico di Milano. The document covers: Grade of written test: Final grade: Grade of project: Signature: Technologies for Sensors and Clinical Instrumentation 16 September 2015 FAMILY NAME ……………………….… NAME ….…………………. MATR ………… PREFERENCE for ORAL: † 17 September (morning) † 22 September (morning) † 6 October (morning)

Technologies for Sensors and Clinical InstrumentationFull exam

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Full exam for Technologies for Sensors and Clinical Instrumentation in the Biomedical Engineering degree programme at Politecnico di Milano. The document covers: Grade of written test: Final grade: Grade of project: Signature: Technologies for Sensors and Clinical Instrumentation 16 September 2015 FAMILY NAME ……………………….… NAME ….…………………. MATR ………… PREFERENCE for ORAL: † 17 September (morning) † 22 September (morning) † 6 October (morning)

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Grade of written test: Final grade: Grade of project: Signature: Technologies for Sensors and Clinical Instrumentation 16 September 2015 FAMILY NAME ……………………….… NAME ….…………………. MATR ………… PREFERENCE for ORAL: † 17 September (morning) † 22 September (morning) † 6 October (morning) † next calls Notes: - Answers are considered complete only if units of measurement are indicated. - Only answers on printed sheets are considered for evaluation. Q.1) (7 points) …/2 - 1.a) Describe the working principle of a Coulter Counter. ……………………………………………………………… ………………………………………..……………………… ……………………………………………………………… ………………..……………………………………………… ………………………………………………………..……… ……………………………………………………………… ………………………………..……………………………… ……………………………………………………………… ………..……………………………………………………… ………………………………………………..……………… ……………………………………………………………… ………………………..……………………………………… ………………………..……………………………………… ……………………………………………………………….. ……………………………………………………………………………………………………….. ……………………………………………………………………………………………………….. ……………………………………………………………………………………………………….. ……………………………………………………………………………………………………….. A constant-voltage Coulter Counter (in figure) is used to compute the size of neutral particles. The particles are inserted in the left compartment and flow through a small aperture (diameter Ø ݉݊ length ൌ ݉݊ ,zeta potential ܸ݉ surface negatively coated). A negative hydraulic pressure of ܽܲܭis applied to the right cell, while the left cell is at atmospheric pressure. A negative voltage is applied to the right cell, equal to ܸ݉ . 1 - 1.b) Total particle velocity within the aperture ݒ௉ is given by the sum of three contributions: a) hydrodynamic flow due to the difference of hydraulic pressure through the apertureݒு௬ௗ௥௢, b) electrophoretic flow ݒா௉ and c) electro-osmotic flow ݒாை. Which one among these contributions is negligible?…

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